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@#[Abstract] Objective: To investigate the effect of cytokeratin 13 (CK13) on radio-sensitivity of human nasopharyngeal carcinoma HNE1 cell line and its mechanism. Methods: HNE1 cells were divided into control group, anti-CK13#a group (CK13 knockdown), anti-CK13#b group (CK13 knockdown), control+sirolimus group (100 nmol/L sirolimus treatment for 1 h), and anti-CK13#a + sirolimus group (100 nmol/L sirolimus treatment for 1 h). After irradiation treatment (200 cGy/min irradiation for 5 min), cell proliferation in each group was measured by CCK-8 assay. Cell apoptosis rate in each group was determined by Flow cytometry. Expression of PI3K/AKT/mTOR signaling pathway related PTEN gene was detected by qPCR, and WB was used to detect the expressions of PI3K/AKT/mTOR signaling pathway related proteins. Results: In the case of radiotherapy, as compared with the control group, the proliferation of HNE1 cells after CK13 knockdown was significantly enhanced (P<0.01) while the apoptosis rate was significantly reduced (P<0.01), the contents of caspase-3 and γH2AX as well as the protein lever of PTEN in cells were significantly decreased, while the expressions of p-AKT and p-S6K were significantly increased (all P<0.01). Interestingly, additional treatment with sirolimus (PI3K/AKT/mTOR signaling pathway inhibitor) could rescue the accelerated cell proliferation and decreased cell apoptosis caused by CK13 knockdown (all P<0.05). Conclusion: CK13 knockdown can enhance the activity of PI3K/AKT/mTOR signaling pathway by down-regulating PTEN, and ultimately reduce the radio-sensitivity of nasopharyngeal carcinoma HNE1 cells.
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@#[Abstract] Objective： ：To investigate the effect of miRNA-325-3p and its target gene cytokeratin 13 (CK13) on the radio-sensitivity of nasopharyngeal carcinoma cell line CNE1. Methods： ：The potential target gene of miRNA-325-3p was predicted by three databases: miRBase, Targetscan and microcosm, and verified by Double luciferase activity assay. QPCR was used to detect the expression levels of miRNA-325-3p and its target gene in nasopharyngeal carcinoma cell line CNE1 under different radiation doses; To verify the changes in radio-sensitivity of nasopharyngeal carcinoma cells, colony formation assay, Flow cytometery and MTT were used to observe the clone formation, apoptosis and cell viability of CNE1 cells after overexpression of miRNA-325-3p and knockdown of CK13 under different radiation doses, respectively. Results： ：CK13 was confirmed as a potential target gene of miRNA-325-3p. After radiotherapy, the expression level of miRNA-325-3p in CNE1 cell was significantly increased, while the expression level of CK13 was decreased (all P<0.05); up-regulation of miRNA-325-3p expression and silence of CK13 gene increased cell survival rate (upregulation of miRNA325-3p: [60.14±3.55]% vs [19.23±3.42]%, t=14.37, P<0.01; silence of CK13: [76.15±5.13]% vs [28.53±3.68]%, t=13.06, P<0.01) and colony formation rate, and decreased apoptosis rate (upregulation of miRNA-325-3p: [27.95±2.67]% vs [51.68±3.47]%, t=9.39, P<0.01; silence of CK13: [20.31±2.62]% vs [38.14±3.83]%, t=6.66, P<0.01). Conclusion： ：miRNA-325-3p can reduce the sensitivity of nasopharyngeal carcinoma cell line CNE1 to radiotherapy by down-regulating the target gene CK13.
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Objective@#To investigate factors associated with awareness of prevention and control of Norovirus outbreak among primary and middle school staffs in the Pearl River Delta region，to provide evidence for Norovirus outbreaks prevention and control.@*Methods@#A total of 320 primary and middle school staffs from 48 schools in 3 cities in the pearl river delta region were selected by multistage quota random sampling method and were interviewed face to face with questionnaire about knowledge of Norovirus and prevention and control of Norovirus outbreaks.@*Results@#The awareness rate of Norovirus knowledge and prevention and control of Norovirus outbreaks among primary middle school and the total school staffs in the pearl river delta region were 35.37%,57.63% and 48.42%,respectively. The results of univariate analysis showed that the awareness rate of prevention and control of Norovirus outbreaks was significantly affected by duties, the total awareness rate among teachers(OR =0.22，95%CI=0.09-0.55),cooks(OR=0.09，95%CI=0.04-0.23)and cleaners(OR=0.02，95%CI=0.00-0.07)were far lower than school doctors(P<0.05).@*Conclusion@#The awareness of prevention and control of Norovirus outbreak among primary and middle school staffs in the Pearl River Delta region was needed to be improved. Valid implements, such as operation procedure, to build up prevention and control capability should be carried out that focus on specific job responsibilities.
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Objective To explore the effects of nursing intervention based on domiciliary oxygen therapy tracking tables (DOTT) quantitative classification on domiciliary oxygen in elderly patients with stable chronic obstructive pulmonary disease (COPD).Methods Forty-two cases of elderly patients with stable COPD treated by domiciliary oxygen therapy from January 2015 to March 2015 were selected as the experimental group,and were given bilateral nasal catheter domiciliary oxygen therapy,and were provided domiciliary oxygen therapy management,nursing education and intervention according to results of family oxygen therapy compliance,symptom identification and oxygen safety,and family oxygen therapy confidence evaluated by family oxygen therapy tracking table.Other 30 cases of patients with stable COPD treated by bilateral nasal catheter domiciliary oxygen therapy and routine nursing during the same period of time were selected as the control group.Both groups were provided nursing care for at least 6 months.Nursing adherence,acute episode,patient satisfaction,and quality of life score before and after nursing of two groups were compared.Results Compared with the control group,nursing adherence and patient satisfaction after 6 months in the experimental group were higher,while rate of patients with acute episodes ≥ 2 times in the experimental group was lower (P＜0.05).Compared with that before nursing intervention,quality of life scores of two groups after nursing intervention were lower(P＜0.05).Compared with the control group,quality of life scores of the experimental group were all decreased (P＜0.05).Conclusion Nursing intervention based on domiciliary oxygen therapy tracking tables quantitative classification can effectively reduce the incidence of acute episodes,improve patient's nursing compliance,patient satisfaction,and quality of life.